LOCUS       V012713                 9452 bp    DNA     circular SYN 08-APR-2021
DEFINITION  Exported.
ACCESSION   V012713
VERSION     V012713
KEYWORDS    pLVX-TRE3G-Luc Control
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9452)
  AUTHORS   caoheibi
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 9452)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9452
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label="3' LTR"
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1303..1536
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1721..1765
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1914..1955
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    2027..2144
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        2206..2555
                     /label="TRE3GV promoter"
                     /note="3rd-generation Tet-responsive promoter that can be
                     activated by binding of Tet-On(R) 3G, optimized for
                     retroviral and lentiviral vectors"
     CDS             2565..4214
                     /label="luciferase"
                     /note="firefly luciferase"
     promoter        4237..4736
                     /label="PGK promoter"
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             4757..5353
                     /label="PuroR"
                     /note="puromycin N-acetyltransferase"
     misc_feature    5374..5962
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             6170..6803
                     /label="5' LTR"
                     /note="5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6932..6948)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(6956..6972)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6980..7010)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7025..7046)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(7334..7919)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(8093..8950)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(8951..9055)
                     /label="AmpR promoter"
     polyA_signal    9103..9237
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga actacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgagtt tactccctat
     2221 cagtgataga gaacgtatga agagtttact ccctatcagt gatagagaac gtatgcagac
     2281 tttactccct atcagtgata gagaacgtat aaggagttta ctccctatca gtgatagaga
     2341 acgtatgacc agtttactcc ctatcagtga tagagaacgt atctacagtt tactccctat
     2401 cagtgataga gaacgtatat ccagtttact ccctatcagt gatagagaac gtatgtcgag
     2461 gtaggcgtgt acggtgggcg cctataaaag cagagctcgt ttagtgaacc gtcagatcgc
     2521 ctggagcaat tccacaacac ttttgtctta tacttggatc caccatggaa gacgccaaaa
     2581 acataaagaa aggcccggcg ccattctatc cgctagagga tggaaccgct ggagagcaac
     2641 tgcataaggc tatgaagaga tacgccctgg ttcctggaac aattgctttt acagatgcac
     2701 atatcgaggt gaacatcacg tacgcggaat acttcgaaat gtccgttcgg ttggcagaag
     2761 ctatgaaacg atatgggctg aatacaaatc acagaatcgt cgtatgcagt gaaaactctc
     2821 ttcaattctt tatgccggtg ttgggcgcgt tatttatcgg agttgcagtt gcgcccgcga
     2881 acgacattta taatgaacgt gaattgctca acagtatgaa catttcgcag cctaccgtag
     2941 tgtttgtttc caaaaagggg ttgcaaaaaa ttttgaacgt gcaaaaaaaa ttaccaataa
     3001 tccagaaaat tattatcatg gattctaaaa cggattacca gggatttcag tcgatgtaca
     3061 cgttcgtcac atctcatcta cctcccggtt ttaatgaata cgattttgta ccagagtcct
     3121 ttgatcgtga caaaacaatt gcactgataa tgaactcctc tggatctact gggttaccta
     3181 agggtgtggc ccttccgcat agaactgcct gcgtcagatt ctcgcatgcc agagatccta
     3241 tttttggcaa tcaaatcatt ccggatactg cgattttaag tgttgttcca ttccatcacg
     3301 gttttggaat gtttactaca ctcggatatt tgatatgtgg atttcgagtc gtcttaatgt
     3361 atagatttga agaagagctg tttttacgat cccttcagga ttacaaaatt caaagtgcgt
     3421 tgctagtacc aaccctattt tcattcttcg ccaaaagcac tctgattgac aaatacgatt
     3481 tatctaattt acacgaaatt gcttctgggg gcgcacctct ttcgaaagaa gtcggggaag
     3541 cggttgcaaa acgcttccat cttccaggga tacgacaagg atatgggctc actgagacta
     3601 catcagctat tctgattaca cccgaggggg atgataaacc gggcgcggtc ggtaaagttg
     3661 ttccattttt tgaagcgaag gttgtggatc tggataccgg gaaaacgctg ggcgttaatc
     3721 agagaggcga attatgtgtc agaggaccta tgattatgtc cggttatgta aacaatccgg
     3781 aagcgaccaa cgccttgatt gacaaggatg gatggctaca ttctggagac atagcttact
     3841 gggacgaaga cgaacacttc ttcatagttg accgcttgaa gtctttaatt aaatacaaag
     3901 gataccaggt ggcccccgct gaattggagt cgatattgtt acaacacccc aacatcttcg
     3961 acgcgggcgt ggcaggtctt cccgacgatg acgccggtga acttcccgcc gccgttgttg
     4021 ttttggagca cggaaagacg atgacggaaa aagagatcgt ggattacgtc gccagtcaag
     4081 taacaaccgc caaaaagttg cgcggaggag ttgtgtttgt ggacgaagta ccgaaaggtc
     4141 ttaccggaaa actcgacgca agaaaaatca gagagatcct cataaaggcc aagaagggcg
     4201 gaaagtccaa attgtaaaat acgcgtgaat tctaccgggt aggggaggcg cttttcccaa
     4261 ggcagtctgg agcatgcgct ttagcagccc cgctgggcac ttggcgctac acaagtggcc
     4321 tctggcctcg cacacattcc acatccaccg gtaggcgcca accggctccg ttctttggtg
     4381 gccccttcgc gccaccttct actcctcccc tagtcaggaa gttccccccc gccccgcagc
     4441 tcgcgtcgtg caggacgtga caaatggaag tagcacgtct cactagtctc gtgcagatgg
     4501 acagcaccgc tgagcaatgg aagcgggtag gcctttgggg cagcggccaa tagcagcttt
     4561 gctccttcgc tttctgggct cagaggctgg gaaggggtgg gtccgggggc gggctcaggg
     4621 gcgggctcag gggcggggcg ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc
     4681 ttcaaaagcg cacgtctgcc gcgctgttct cctcttcctc atctccgggc ctttcgacct
     4741 gcagcccaag cttaccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga
     4801 cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca
     4861 caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac
     4921 gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt
     4981 ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat
     5041 ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc
     5101 gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca
     5161 gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg
     5221 ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg
     5281 cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg
     5341 caagcccggt gcctgacggg gcgcgtctgg aacaatcaac ctctggatta caaaatttgt
     5401 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     5461 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     5521 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     5581 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     5641 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     5701 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     5761 tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc
     5821 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     5881 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     5941 tccctttggg ccgcctcccc gcctggaatt aattctgcag tcgagaccta gaaaaacatg
     6001 gagcaatcac aagtagcaat acagcagcta ccaatgctga ttgtgcctgg ctagaagcac
     6061 aagaggagga ggaggtgggt ttttccagtc acacctcagg tacctttaag accaatgact
     6121 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa agaggggact ggaagggcta
     6181 attcactccc aacgaagaca agatatcctt gatctgtgga tctaccacac acaaggctac
     6241 ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact gacctttgga
     6301 tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa taaaggagag
     6361 aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagtg
     6421 ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga gctgcatccg
     6481 gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc tggggacttt
     6541 ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc ctgcatataa
     6601 gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct
     6661 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     6721 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     6781 gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata
     6841 acttgcaaag aaatgaatat cagagagtga gaggccttga cattgctagc gttttaccgt
     6901 cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     6961 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     7021 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     7081 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     7141 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     7201 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     7261 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     7321 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     7381 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     7441 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     7501 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     7561 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     7621 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     7681 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     7741 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     7801 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     7861 ctggtagcgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     7921 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     7981 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     8041 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     8101 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     8161 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     8221 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     8281 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     8341 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     8401 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     8461 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     8521 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     8581 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     8641 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     8701 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     8761 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     8821 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     8881 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     8941 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     9001 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     9061 ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tcaacttgtt tattgcagct
     9121 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     9181 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcaac
     9241 tggataactc aagctaacca aaatcatccc aaacttccca ccccataccc tattaccact
     9301 gccaattacc tagtggtttc atttactcta aacctgtgat tcctctgaat tattttcatt
     9361 ttaaagaaat tgtatttgtt aaatatgtac tacaaactta gtagttttta aagaaattgt
     9421 atttgttaaa tatgtactac aaacttagta gt
//
